Research on the A549 cell apoptosis mechanism of the nude mouse model using MenSC-sTRAIL

H. Ding, J. Wang, F.-P. Jia, J. Yi, M. Zhang

Department of Respiratory Diseases, Affiliated People’s Hospital of Jiangsu University, Zhenjiang, Jiangsu Province, China. wangjian160908@163.com

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• Oncology

OBJECTIVE: To investigate the inhibitory effect of MenSC-sTRAIL on the apoptosis of A549 cells of lung cancer in a nude mouse model and the relevant molecular mechanism.

MATERIALS AND METHODS: The tumorigenesis curve of A549 cells in the nude mouse model was assayed using the growth curve plotted by the growth rate of tumor of the A549 cell nude mouse model. Cellular apoptosis was assayed using flow cytometer and Annexin V-FITC, and the effect of MenSC-sTRAIL on the expression of Bax was measured using Western blot.

RESULTS: MenSC-sTRAIL promoted the apoptosis of A549 cells. Results of the quantitative assay using Annexin V-FITC further confirmed the effect of MenSC-sTRAIL on inducing the cellular apoptosis. Western-blot results showed that MenSC-sTRAIL increased the expression of Bax.

CONCLUSIONS: MenSC-sTRAIL may vary the balance of regulation of heterodimer of Bcl-2 and Bax, which can induce the apoptosis of A549 cells.

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