MiR-222 promotes proliferation, migration and invasion of lung adenocarcinoma cells by targeting ETS1

Q. Sun, C.-W. Jiang, Z.-H. Tan, L.-Y. Hou, H. Dong, K. Liu, G. Sun, Y.-J. Liu, Y.-Q. Wang, X.-C. Lu, H. Liu

Department of Intensive Care Unit, Affiliated Hospital of Qingdao University, Qingdao, Shandong, China. wn61200008ziwei@163.com

---

• Oncology

OBJECTIVE: The lung adenocarcinoma is a type of lung cancer. This research is to investigate the effects of miR-222 on the proliferation, migration and invasion of the lung adenocarcinoma cells.

MATERIALS AND METHODS: At the beginning, MiR-222 and the controls were transfected to the lung adenocarcinoma cell line A549 for CCK-8 proliferation, transwell migration and Matrigel invasion, and then observed the effect of miR-222 on the proliferation, migration and invasion of lung adenocarcinoma cells. The miR-222 target was regulated by ETS1 downwards to participate in the regulation of the process by using the luciferase reporter assay, the Real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) and the Western blotting.

RESULTS: According to CCK-8 proliferation assay, the Transwell migration and the Matrigel invasion assay, it discovered that MiR-222 can promote the proliferation, migration and invasion of the lung adenocarcinoma cells. Luciferase reporter assay, RT-qPCR and Western blot assay showed that miR-222 could regulate the expression of ETS1 downwards and ETS1 participated in the regulation of the process

CONCLUSIONS: ETS1 promotes proliferation, migration and invasion of lung adenocarcinoma cells by targeting the regulated miR-222 downwards.

Free PDF Download