TGF-β1 promotes cells invasion and migration by inducing epithelial mesenchymal transformation in oral squamous cell carcinoma

J.-Q. Bu, F. Chen

Department of Stomatology, Chinese PLA General Hospital, Beijing, China. 25852832@qq.com

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• Oncology

OBJECTIVE: To investigate the role of TGF-β1 on epithelial mesenchymal transition (EMT) and invasion in oral squamous cell carcinoma cell line Tca8113.

MATERIALS AND METHODS: Cultured Tca8113 cells were treated with different concentrations of TGF-β1 for 24 h. The morphological changes were observed under phase-contrast microscopy. The mRNA and protein expression levels of EMT relative marker E-cadherin and Vimentin were detected by RT-PCR and Western blot. The effect of TGF-β1 on migration and invasion ability of Tca8113 cells were detected using transwell method.

RESULTS: The results demonstrated that TGF-β1 could induce morphological changes in Tca8113 cells from epithelial to mesenchymal. The mRNA and protein level of epithelial marker E-cadherin was downregulated following treatment with TGF-β1, whereas the mRNA and protein expression level of mesenchymal marker protein Vimentin was upregulated. Furthermore, TGF-β1 significantly enhances the migration and invasiveness of Tca8113 cells, which were effectively reversed by TGF-β1 inhibitor, LY2109761

CONCLUSIONS: TGF-β1 enhances Tca8113 cells migration and invasion by inducing epithelial mesenchymal transition.

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