MicroRNA-200c represses migration and invasion of gastric cancer SGC-7901 cells by inhibiting expression of fibronectin 1

H.-B. Chen, H.-T. Zheng

Department of Gastrointestinal Surgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical College, Yantai, China. chb2087@sina.com

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• Oncology

OBJECTIVE: Gastric cancer remains a worldwide burden as a leading cause of cancer-related death. Drug resistance of chemotherapy looms as a major clinical challenge to effective treatment. Recent research data has suggested that microRNAs could be a potential gastric cancer treatment strategy. To further evaluate the role of microRNAs on gastric cancer cells and its underlying possible mechanism, we transfected human gastric cancer SGC-7901 cells with microRNA-200c.

MATERIALS AND METHODS: The cell proliferation, migration and invasion of SGC-7901 were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, Transwell assay and cell invasion assay. The expression of FN1 was detected by the reverse transcriptase-polymerase chain reaction (RT-PCR) assay.

RESULTS: The cell proliferation, migration and invasion were all significantly decreased after microRNA-200c transfection. Moreover, Fibronectin 1 (FN1) expression was significantly inhibited by microRNA-200c transfection. These results indicated that the mechanism by which microRNA-200c impresses human gastric cancer SGC-7901 cells may be mediated by its inhibition on FN1 expression.

CONCLUSIONS: This study highlighted the potential of using microRNA-200c as a new treatment strategy for human gastric cancer.

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