MiRNA153 induces pituitary tumor MMQ cell line apoptosis through down-regulating Skp protein expression

Z.-R. Zhao, M. Li, P. Shi, P. Zhang

Department of Neurology, Yantai Yeda Hospital, Yantai, Shandong, China. pengzhangcvb@163.com

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• Oncology

OBJECTIVE: Pituitary tumor seriously threatens patient’s life. MicroRNAs regulate cell growth and apoptosis. This study aims to investigate the effects of miRNA153 on pituitary tumor MMQ cells proliferation and apoptosis.

MATERIALS AND METHODS: Synthetic miRNA153 and control miRNA were transfected to MMQ cell line. Cell proliferation and apoptosis were tested by MTT assay and flow cytometry, respectively. Skp protein expression was detected by Western blot assay. Skp siRNA or Skp plasmid was transfected to MMQ cells transfected by miRNA153 to evaluate the influence on MMQ cell apoptosis.

RESULTS: MMQ cell proliferation was inhibited and apoptosis was enhanced after miRNA153 transfection. Skp protein level decreased in MMQ cells transfected by miRNA153. Skp interference enhanced MMQ cell apoptosis induced by miRNA153. Skp overexpression restrained MMQ cell apoptosis triggered by miRNA153.

CONCLUSIONS: MiRNA153 transfection suppressed MMQ cell growth and induced apoptosis. MiRNA153 regulated MMQ cell apoptosis through down-regulating Skp protein.

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