GC/MS-based metabolomic analysis of alleviated renal ischemia-reperfusion injury induced by remote ischemic preconditioning

S. Shen, J.-F. Wang, J.-Q. Wu, J.-X. Zhou, S.-D. Meng, J. Ma, C.-L Zhu, G.-G. Deng, D. Liu

Department of Organ Transplantation, Guangdong No. 2 Provincial People’s Hospital, Guangzhou, China. shsh0844@aliyun.com

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• Nephrology

OBJECTIVE: Dysfunctional metabolisms have contributed towards ischemia-reperfusion (I/R) injury. However, the role of remote ischemic preconditioning (RIP) in I/R injury is not well known. The present study showed alleviated I/R injury in kidneys treated with RIP.

MATERIALS AND METHODS: We utilized GC/MS-based metabolomics to characterize the variation of metabolomes.

RESULTS: Metabolic category using differential metabolites showed the lower percentage of amino acids in I/R group in comparison to RIP+I/R group, confirming the importance of amino acid metabolism in RIP-treated rat kidney. Further, pathway enrichment analysis showed alanine, aspartate and glutamate metabolism to be involved in the beneficial effects of RIP during renal I/R injury. Furthermore, another crucial enrichment pathway is biosynthesis of unsaturated fatty acids. Other vital metabolites detected in independent component analysis (ICA) analysis were d-glucose, lactic acid and cholesterol. The variation tendency of above-mentioned metabolites was overall consistent with the protective nature of RIP.

CONCLUSIONS: These findings elicited a viewpoint that metabolic strategy affected by RIP are linked to underlying mechanisms of RIP and highlighted the importance of metabolic strategy against I/R injury.

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