The expression and function of miRNA-106 in pediatric osteosarcoma

M. Xu, Y.-Y. Zhang, H.-F. Wang, G.-S. Yang

Department of Pediatric Surgery, Yishui Central Hospital of Linyi City, Yishui, Shandong Province, P.R. China. yangguang1929@sina.co

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• Oncology

OBJECTIVE: This study is to investigate the expression and biological function of miRNA-106b in osteosarcoma.

PATIENTS AND METHODS: Freshly resected osteosarcoma tissues and the corresponding para-carcinoma tissues were collected from 54 patients. Then miR-106b level in the carcinoma tissues was detected by real-time PCR. To test the function of miR-106b, osteosarcoma cell line U2-OS was transfected with miR-106b inhibitor in vitro. Then, the proliferation, cell cycle, invasion and metastasis of U2-OS cells were detected by CCK-8 assay, flow cytometry, and transwell respectively. The PI3K/AKT signaling pathway activity after treatment was detected by Western blot.

RESULTS: miR-106b level was significantly higher in osteosarcoma, and its expression was positively correlated with the lung metastasis and the clinical stages. In vitro experiments showed that the proliferation, invasion, and migration of U2-OS osteosarcoma cells were inhibited after miR-106b inhibition. The transition from G1 to S phase of U2-OS osteosarcoma cells was inhibited after miR-106b inhibition. The Western blot analysis demonstrated that both the AKT phosphorylation in PI3K/AKT pathway and the PI3Kp100 expression were significantly reduced when the expression of miR-106b was down-regulated.

CONCLUSIONS: miR-106b level was significantly up-regulated in osteosarcoma, which was positively correlated with the lung metastasis and clinical stages. The down-regulation of miR-16b inhibited the proliferation, invasion, and migration of osteosarcoma cells; thus, it may function as an oncogene to promote osteosarcoma proliferation and invasion through the PI3K/AKT signaling pathway.

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