Research of the relationship between β-catenin and c-myc-mediated Wnt pathway and laterally spreading tumors occurrence

L. Shi, Y.-X. Wu, J.-H. Yu, X. Chen, X.-J. Luo, Y.-R. Yin

Department of Gastroenterology, The Affiliated Hospital of Southwest Medical University, Sichuan Province, Luzhou China. eql9688767@163.com

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• Oncology

OBJECTIVE: We studied the relationship between the occurrence of laterally spreading tumors (LSTs) and the activation of Wnt/β-catenin/c-myc pathway.

PATIENTS AND METHODS: 20 cases of colorectal polypoid adenomas (PAs) tissue sample, 20 cases of LSTs tissue sample and 20 cases of colorectal cancer tissue sample were acquired, and positive expression of Wnt/β-catenin/c-myc proteins were detected by immunohistochemical staining. In addition, relative expression levels of mRNA and protein were measured using RT-PCR and Western blotting method.

RESULTS: The positive expression levels of Wnt/β-catenin/c-myc protein in the colorectal cancer group were higher than that in LST group, and the value of the PAs group was the lowest; differences were statistically significant (p < 0.05). The relative expression levels of Wnt/β-catenin/c-myc mRNA and protein in colorectal cancer group were higher than that in LST group, and the value of PAs group was the lowest; differences were statistically significant (p < 0.05).

CONCLUSIONS: The activation level of the Wnt/β-catenin/c-myc pathway has a close relation to the occurrence and deterioration of LSTs.

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