MiR-508-5p is a prognostic marker and inhibits cell proliferation and migration in glioma

Y.-H. Liu, B. Li, F.-G. Meng, L. Qiu

Department of Neurosurgery, Linyi People’s Hospital, Linyi, Shandong, China. nn05nn1983@year.com

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• Oncology

OBJECTIVE: Increasing evidence has informed that dysregulation of microRNAs (miRNAs) may contribute to carcinogenesis in human. The aim of the present study was to determine the role of miR-508-5p in glioma.

PATIENTS AND METHODS: Quantitative real-time PCR was performed to detect the expression levels of miR-508-5p in glioma and normal control tissues. In vitro, migration assays and a wound-healing assay were performed to determine the effects of miR-508-5p. Associations between miR-508-5p expressions and various clinicopathological characteristics were analyzed. Survival rate was determined with Kaplan-Meier. Univariate and multivariate analyses were performed to estimate the effects of the expression of miR-508-5p on survival.

RESULTS: The expression of miR-508-5p was downregulated in glioma tissues and cell lines. Low miR-508-5p expression was related to WHO grade (p = 0.005) and KPS score (p = 0.013). Moreover, Kaplan-Meier survival analysis showed that low miR-508-5p expression was significantly associated with short overall survival (p = 0.0059). Furthermore, multivariate analysis revealed that miR-508-5p was an independent prognostic factor for the overall survival in glioma (p = 0.002). Finally, forced expression of miR-508-5p could inhibit glioma cell growth and metastasis in vitro.

CONCLUSIONS: Taken together, our study suggested that miR-508-5p may be served as a novel prognostic factor and may lead to new treatment strategies for glioma.

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