Evaluation of correlation between NF-κB mediated PAI-1 gene and sepsis

L.-X. Wang, L.-M. Tang, G.-Q. Pan, C.-X. Yan, L. Lu, Y.-F. Liang

Department of Pediatric ICU, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, P. R. China. wztang0577@163.com

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• Infectious Diseases

OBJECTIVE: The present study is aimed to explore the correlation between PAI-1 gene mediated by NF-κB signaling pathway and human sepsis.

PATIENTS AND METHODS: In this study, we used 74 cases of sepsis patients preserved by the laboratory as the observation group, and 68 cases of healthy people served the control group. Further methods like fluorescence quantitative PCR, enzyme-linked immunosorbent assay, Western-blotting were used to determine NF-B expression, NF-κB gene mRNA analyses and protein expression on different research subjects. Further, the positive expression rates of PAI-1 gene in the observation group and the control group were determined by immunohistochemistry.

RESULTS: The expression levels of NF-κB and PAI-1 gene mRNA in the blood of the observation group significantly increased in comparison to control group (X2 = 3.24, p < 0.05; X2 = 2.81, p < 0.05). Also, NF-κF and PAI-1 gene protein expressions (0.14 ug/l, 0.32 ug/l) were significantly higher in the observation group in comparison to control group (p < 0.05). The results of blood glucose measurement showed that the fasting blood glucose (14.3 mmol/l) in the observation group was significantly higher than that in the control group (4.6 mmol/l). Immunohistochemical were also in sync with above results.

CONCLUSIONS: The present study concludes that PAI-1 gene expression gets significantly increased via NF-κF signaling pathway during sepsis.

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