Long non-coding RNA MVIH acts as a prognostic marker in glioma and its role in cell migration and invasion

J.-J. Zhuang, M. Yue, Y.-H. Zheng, J.-P. Li, X.-Y. Dong

Department of Neurology, Weifang People’s Hospital, Weifang, Shandong, China. j0901jop@163.com

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• Oncology

OBJECTIVE: High expression levels of lncRNA associated with microvascular invasion in HCC (lncRNA MVIH) were found to correlate with several solid tumors. However, little is known concerning the function of MVIH in glioma. The purpose of our study is to explore the role of lncRNA MVIH in clinical glioma samples and cell lines.

PATIENTS AND METHODS: The expression levels of MVIH were analyzed in glioma surgical resection tissues and cells by RT-PCR. Additionally, the associations of MVIH expression with clinicopathological features were analyzed. Survival and Cox proportional-hazards regression analyses were performed to determine the correlation between MVIH expression levels and prognosis in the patients. The cell proliferation, migration ability, invasion ability were measured successively by CKK-8 assay, transwell and wound healing assay.

RESULTS: We found that MVIH was significantly upregulated in glioma cell lines and tissues. Furthermore, MVIH expression was positively correlated with KPS and WHO grade. Patients with MVIH high expression tumors had a worse overall survival compared to patients with MVIH high expression tumors. Moreover, Univariate and multivariate Cox regression analysis confirmed that MVIH was an independent risk factor for glioma. Finally, in vitro, we showed that up-regulation of MVIH expression promoted human glioma cells proliferation, invasion and migration, while down-regulation of MVIH expression suppressed human glioma cells proliferation, invasion and migration.

CONCLUSIONS: Our findings indicated that MVIH expression may serve not only as a prognostic marker but also as a potential therapeutic target in glioma.

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