MicroRNA-142 promotes the expression of eNOS in human peripheral blood-derived endothelial progenitor cells in vitro

H.-W. Zhang, H. Li, H. Yan, B.-L. Liu

Department of Neurosurgery, Second Hospital of Tianjin Medical University, Tianjin China. hua_yan2008@163.com

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• Pharmacology

OBJECTIVE: MicroRNAs (miRNAs) are small non-coding RNAs (18-25nt) that regulate gene expression mainly through affecting post-transcriptional modification. In this research, the human peripheral blood-derived endothelial progenitor cells were isolated to research the inter-coordination of miR-142, ADAMTS-1, VEGF and eNOS through gene over-expressed in EPCs.

MATERIALS AND METHODS: Endothelial progenitor cells (EPCs) were isolated from human peripheral blood, and demonstrate the interaction of miR-142 and targets (ADAMTS-1) in vitro.

RESULTS: The results showed that miR-142 could promote the productivity of NO through inhibited ADAMTS-1 expression (inhibitor of VEGF), activated function of VEGF and eNOS in human EPCs. This finding suggests that miR-142 and eNOS may have cooperative effect in vessel tone and play an important role in the angiogenesis.

CONCLUSIONS: In our research, we demonstrated that the miR-142 can promote eNOS expression through down-regulated ADAMTS-1 expression (inhibitor of VEGF) in human EPCs, these results provide a new insight in microRNA regulation of vessel tone and angiogenesis.

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