miR-494 inhibits invasion and proliferation of gastric cancer by targeting IGF-1R

X.-Q. Zhao, T.-J. Liang, J.-W. Fu

Department of Digestive Diseases, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong Province, PR China. zhaopml01@sina.com

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• Oncology

OBJECTIVE: The aim of this study was to investigate the target gene of miR-494 and its roles in tumor growth of gastric cancer (GC).

PATIENTS AND METHODS: Expression of miR-494 was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR) in gastric cancer tissues and cell lines. Then, luciferase reporter assay was used to elucidate whether insulin-like growth factor 1 receptor (IGF1R) is a target gene of miR-494. Finally, the roles and mechanism of miR-494 in the regulation of tumor invasion were further investigated.

RESULTS: Relative miR-494 level was found to be significantly lower in patients with GC than healthy controls (p < 0.01). Over-expression of miR-494 could inhibit gastric cancer cell proliferation, migration, and invasion in vitro. Furthermore, we demonstrated that miR-494 binds to the 3′-untranslated region (UTR) of IGF1R and inhibits the expression of the IGF1R protein.

CONCLUSIONS: Our data showed that miR-494 acted as a tumor suppressor in GC.

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