MiR-200c and miR-141 inhibit ZEB1 synergistically and suppress glioma cell growth and migration

E. Guo, Z. Wang, S. Wang

Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, China. shuxingwang1@outlook.com

---

• Oncology

OBJECTIVE: This study aimed to investigate the expression of miR-200c and miR-141 in glioma tissues and cell lines and then to study their regulative effect on ZEB1 expression and on glioma cell growth and migration.

MATERIALS AND METHODS: QRT-PCR analysis was performed to detect miR-200c and miR-141 expression in 10 paired glioma tissues and adjacent normal tissues from patients with glioblastoma multiforme (GBM) and in glioma cell lines. U87 and U251 cells were transfected with miR-200c mimics, miR-141 mimics or ZEB1 siRNA respectively. ZEB1 expression was detected qRT-PCR and Western blot assay. MTT assay, flow cytometry and wound healing assay were performed to examine the tumor suppressive effects of the miR-200c/miR-141-ZEB1 axis on glioma cells.

RESULTS: Both miR-200c and miR-141 were significantly lower in glioma tissues than in adjacent normal tissues. The glioma cell lines, including U87, U251 and A172 also had significantly decreased miR-200c and miR-141 expression than normal tissues. ZEB1 expression had at least two-fold increase in glioma tissues than in normal tissues. Both miR-200c and miR-141 could significantly induce ZEB1 mRNA degradation and suppress ZEB1 protein expression. ZEB1 siRNA presented similar growth and migration inhibiting and apoptosis inducing effect to miR-200c and miR-141 mimics in U87 cells.

CONCLUSIONS: MiR-200c and miR-141 are significantly downregulated in glioma tissues and cell lines and can significantly induce ZEB1 mRNA degradation and suppress ZEB1 protein expression in the cells. ZEB1 is a functional downstream target of miR-200c and miR-141 in inhibiting glioma cell growth and migration.

Free PDF Download