Metastasis associated in colon cancer 1 (MACC1) promotes growth and metastasis processes of colon cancer cells

J. Tang, J.-X. Chen, L. Chen, J.-Y. Tang, Z. Cui, C.-H. Liu, Z. Wang

Department of General Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China. wangzhmd@163.com

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• Oncology

OBJECTIVE: Colon cancer (CC) is the third most common malignancy with high mortality rate in the world. The impacts of metastasis associated in colon cancer 1 (MACC1) on growth and metastasis processes of CC were investigated by overexpression and interference lentivirus infection.

MATERIALS AND METHODS: Firstly, overexpression and interference plasmids were constructed with GV115 vector and lentivirus were packaged using 293T cells. Human CC cell lines SW1116 and HCT116 were used and divided into four groups respectively, namely control group, blank group, MACC1 siRNA group (infected with interference lentivirus) and MACC1 group (infected with overexpression lentivirus). Then, cell proliferation and clone formation were examined. Besides, migration and invasion of cells were investigated by wound healing and transwell assays, respectively. Additionally, liver metastasis was evaluated in nude xenografts model.

RESULTS: Overexpression and interference lentivirus of MACC1 were successfully constructed. After infected with overexpression lentivirus, cell proliferation, clone formation number, cell invasion and migration capabilities of CC cells were significantly increased (p < 0.05). Furthermore, decreased cell proliferation, smaller and fewer clones, as well as weakened cell migratory and invasive capabilities were observed in SW1116 and HCT116 cells treated with interference lentivirus of MACC1 (p < 0.05). Additionally, liver metastasis rate was higher in SW1116 cells with a higher expression level of MACC1 than that in HCT116 cells with a lower MACC1 expression level.

CONCLUSIONS: MACC1 promotes the growth and metastasis processes of CC cells.

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