The localization of SP-B and influences of lipopolysaccharide on it

W.-N. Wang, J.-H. Zhou, P. Wang, X.-J. Zhang

State Key Laboratory of Trauma, Burns and Combined Injury, Department 4, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, China. traumazjh@126.com

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• Respiratory Medicine

OBJECTIVE: Surfactant protein B (SP-B) is the only essential protein component of lung surfactant, the relationship between localization and function is very close. The localization of rat SP-B and influences of lipopolysaccharide (LPS) on it were investigated.

MATERIALS AND METHODS: The localization of SP-B protein in lung tissue was detected by immunofluorescence after challenged with intratracheal instillation of LPS. The constructed recombinant plasmid pEGFP-N2-SP-B was transfected with CCl-149 cell lines by liposome to explore the localization of SP-B in alveolar type II epithelial cells. Furthermore, the expression and function of SP-B were analyzed with LPS stimulating.

RESULTS: The proteins SP-B and SP-C showed the same pattern of expression in normal lung, and distributed in the alveolar cavity, LPS can directly affect the expression of SP-B in vivo, contrast to SP-B, the LPS-induced influences on SP-C is little. SP-B had the membrane embedded features and localized on the cell surface of CCL149 in vitro, but was influenced by LPS.

CONCLUSIONS: It is confirmed that the expression and membrane function of SP-B were decreased by stimulating LPS in lung tissue and alveolar type II epithelial cells.

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