Endothelial microparticles induce vascular endothelial cell injury in children with Kawasaki disease

J. Tian, H.-T. Lv, X.-J. An, N. Ling, F. Xu

Department of Cardiology, Xuzhou Children’s Hospital, Xuzhou, Jiangsu, P.R. China. tal0979@163.com

---

• Cardiology

OBJECTIVE: To explore the role of microparticles produced by endothelial cells in the injury of vascular endothelial cells.

MATERIALS AND METHODS: We stimulated human umbilical vein endothelial cells (HUVEC) with TNF-α in vitro, analyzed the change of cellular morphology, and measured EMP level in the supernatant with a flow cytometer. Then, we evaluated the corresponding clinical indicators and the role of EMP in endothelial injury.

RESULTS: The endothelial cellular morphology underwent significant changes, and a large number of microparticles were secreted. In turn, these microparticles blocked cell cycle and induced apoptosis.

CONCLUSIONS: The microparticles produced by endothelial cells play an important role in the injury of vascular endothelial cells.

Free PDF Download