Effects of hemodialysis and hemoperfusion on inflammatory factors and nuclear transcription factors in peripheral blood cell of multiple organ dysfunction syndrome

Y.-T. Wang, J.-J. Fu, X.-L. Li, Y.-R. Li, C.-F. Li, C.-Y. Zhou

Intensive Care Unit (ICU), The First Affiliated Hospital of Xinxiang Medical University, Weihui, P.R. China. chenyanzhoucn@163.com

---

• Pharmacology

OBJECTIVE: To investigate the effects of hemodialysis (HD) and hemoperfusion (HP) on inflammatory factors and nuclear transcription factors in peripheral blood cell of multiple organ dysfunction syndrome (MODS) patients.

PATIENTS AND METHODS: 92 cases of MODS patients undergoing maintained hemodialysis in our hospital were randomly divided into the control group and observation group. The control group was treated with conventional hemodialysis (HD), the observation group was treated with hemoperfusion combined therapy (HD+HP) based on the control group. The levels of serum creatinine (SCR), serum total cholesterol (TC), blood urea nitrogen (BUN) and serum albumin (Alb) were compared and analyzed between two groups before and after treatment. The levels of NK-κB p65 in the white cell of peripheral blood were compared between two groups before and after treatment by Western blot. The levels of inflammatory factors TNF-α and IL-6 in peripheral blood were detected between two groups before and after treatment using ELISA method.

RESULTS: Compared with the control group, the levels of Scr, BUN and TC were significantly decreased, while the level of Alb was significantly increased (p < 0.05). The level of cytoplasm NK-κB p65 protein in peripheral blood was significantly increased in the observation group, while the level of nuclear NK-κB p65 in peripheral blood was significantly decreased (p < 0.05).

CONCLUSIONS: Hemodialysis combined with hemoperfusion in treating MODS patients could significantly improve the biochemical indicators, effectively remove the inflammatory mediums, and significantly inhibit the activation of NK-κB.

Free PDF Download