In vitro and in situ antiyeast activity of Gracilaria changii methanol extract against Candida albicans

S. Sasidharan, I. Darah*, K. Jain**

Institute for Research in Molecular Medicine (INFORMM), University Sains Malaysia, Minden, Penang (Malaysia)

*School of Biological Sciences, University Sains Malaysia, Penang (Malaysia)

**School of Chemical Sciences, University Sains Malaysia, Minden, Penang (Malaysia)

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• Phytomedicine

Background and Objectives: The Gracilaria (G.) sp are widely used in the traditional medicine in Malaysia. The methanol extract of Gracilaria changii B.M. Xia & I.A. Abbott (Gracilariaciae) was evaluated for antiyeast activity against Candida albicans (Berkhout).

Materials and Methods: The antiyeast activities were studied by using disc diffusion method and broth dilution method. The effect of the extract on the growth profile of the yeast was also examined via time-kill assay. In addition, the in situ antiyeast activity was studied by microscopic observations using scanning electron microscopy (SEM), transmission electron microscopy (TEM) to determine the major alterations in the microstructure of Candida (C.) albicans.

Results and Discussion: The extract showed a favourable antimicrobial activity against C. albicans with a minimum inhibitory concentration (MIC) value of 1.56 mg/mL. The main abnormalities noted from the SEM and TEM studies were the internal shrinkage of cell, disorganization within the cell cytoplasm and complete collapse of the yeast cells after 36 h of exposure to the extract. The time-kill assay suggested that the G. changii extract significantly inhibited C. albicans growth and it also exhibited prolonged antiyeast activity against the C. albicans.

Conclusion: The extract has shown in vitro fungicidal properties against C. albicans and should be investigated for its therapeutic potential.

Corresponding Author: Sreenivasan Sasidharan, Ph.D.; e-mail: srisasidharan@yahoo.com; srisasidharan@usm.my

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