MicroRNA-494 expression in ovarian cancers and its inhibition on cancer cell proliferation and migration

X.-H. Chen, X.-M. Ling, S. Shi

Department of Gynecology, Henan Provincial People’s Hospital, Zhengzhou, China. chenxh1895@163.com

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• Oncology

OBJECTIVE: The aim of this study is to investigate microRNA-494 (miRNA-494) expression in the plasma and tissue of patients with ovarian cancer and to explore its role in the proliferation and migration of ovarian cancer cells.

PATIENTS AND METHODS: Forty ovarian cancer patients admitted in our institution were included in the study group and 30 healthy subjects seeking health check-up during the same period were included in the control group. miRNA-494 expression level in the cancer tissue as well in the paracancerous tissues of ovarian cancer patients were determined using fluorescent quantitative real-time PCR (fq RT-PCR). Meanwhile, plasma level of miRNA-494 was compared between ovarian cancer patients and normal controls. Furthermore, miRNA-494 expression was interfered by introducing miRNA-494 mimic and inhibitor respectively into HO-8910 ovarian cancer cells. Cell proliferation and migration were analyzed using CCK-8 assay and cell invasion assay using Transwell method.

RESULTS: miRNA-494 was significantly increased in ovarian cancer tissues than in paracancerous tissues (p < 0.05). miRNA-494 was significantly higher in the plasma of ovarian cancer patients than that of normal population (p < 0.05). The proliferation and invasion capacity of ovarian cancer cells were significantly reduced in HO-8910 ovarian cancer cells with suppressed expression of miRNA-494 (p < 0.05). However, the proliferation and invasion capacity of ovarian cancer cells were significantly enhanced in HO-8910 ovarian cancer cells with overexpression of miRNA-494 (p < 0.05).

CONCLUSIONS: miRNA-494 expression is increased in ovarian cancer patients, and miRNA-494 is involved in the progression of ovarian cancer by promoting the proliferation and invasion of ovarian cancer cells.

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