Gene expression analysis of ruptured and un-ruptured saccular intracranial aneurysm

L. Chen, J.Q. Wan, J.P. Zhou, Y.L. Fan, J.Y. Jiang

Department of Neurosurgery, Renji Hospital, School  of Medicine, Shanghai Jiaotong University, Shanghai, China. jieqingwan@126.com

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• Neurology

BACKGROUND: Although Saccular intracranial aneurysm (sIA) is the most common type abnormality of all intracranial aneurysms, the biological mechanisms of sIA are not fully understood.

METHODS: We downloaded microarray datasets from Gene Expression Omnibus (GEO) database which includes 11 ruptured intracranial aneurysm samples and 8 unruptured intracranial aneurysm samples. Significant Analysis of Microarray (SAM) was employed to identify the differentially expressed genes (DEGs) between ruptured and unruptured intracranial aneurysms.

RESULTS: We found 2129 genes differentially expressed in rupture sIA, of which 1062 genes up-regulated and 1057 genes down-regulated. Functional analysis demonstrated these genes were significantly associated with inflammatory response, wounding response and defense response. Protein-protein interaction (PPI) analysis revealed that these genes may play important roles in the pathogenesis of sIAs. Results suggested that four transcription factors (TFs) could cooperated with each other, together with several microRNAs play roles in the pathonegensis of ruptured sIAs.

CONCLUSIONS: All of above results indicate the existence of DEGs between ruptured and unruptured sIAs, which regulating the pathogenesis of ruptured sIAs. TFs and microRNAs may also play key roles in ruptured sIAs. This research hints a new thought to the therapy of ruptured sIAs.

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