Research on mechanism of MAPK signal pathway induced by BMSCs for the proteinuria of rat’s kidney, glomerulosclerosis and activity of RAS

Y. Li, Q. Liu, S.-T. Ou, W.-H. Wu, L.-W. Gan

Department of Nephrology, Affiliated Hospital of Southwest Medical University (SiChuan Clinical Research Center for Nephropathy), Luzhou, Sichuan, China. wuweihua8010@swmu.edu.cn

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• Nephrology

OBJECTIVE: The aim of the article was to explore the mechanism of MAPK (Mitogen-activated protein kinase) signal pathway induced by BMSCs (Bone marrow mesenchymal stem cells) for the proteinuria of rat’s kidney, glomerulosclerosis and activity of RAS (Renin angiotensin) system.

MATERIALS AND METHODS: Thirty rats were divided into sham group, FSGS (Focal Segmental Glomerular Sclerosis) group and BMSCs group. The variation of biochemical criterion and protein of rats in the three groups was compared. The variation condition of rats’ kidney and GSI (Glomerular sclerosis index), ECM/GA (Extracellular matrix/glomerular area) was compared. The activity of RAS was analyzed. Finally, the p38 MAPK and p-p38 MAPK protein was compared.

RESULTS: Compared with sham group rats, the SCr, BUN and proteinuria after twenty-four hours in FSGS group was improved. The blood albumin was notably reduced. At the same time, there was evident deterioration in the pathology of nephridial tissue (p<0.05). The biochemical criterion in transplanted BMSCs group was significantly reduced. At the same time, the blood albumin and pathology of nephridial tissue was also improved (p<0.05). The glomerulus in sham group was normal. There was abundant induration for the glomerulus in FSGS group compared with sham group. The relative value of GSI and ECM/GA was higher than in sham group (p<0.05). The relative value of GSI and ECM/GA in BMSCs group was reduced notably compared with FSGS group (p<0.05). The activity of RAS in FSGS group was enhanced. But activity of RAS in BMSCs group was remarkably restrained. The p38 MAPK and p-p38 MAPK protein in FSGS group was significantly increased compared with the other groups (p<0.05). The protein expression in BMSCs group and inhibitor group was restrained (p<0.05).

CONCLUSIONS: The BMSCs could restrain the proteinuria of rat’s kidney and activity of RAS and they were related with the expression of MAPK signal pathway closely.

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