Circ-ABCB10 promotes growth and metastasis of nasopharyngeal carcinoma by upregulating ROCK1

Z.-N. Duan, C.-G. Dong, J.-H. Liu

Department of Otolaryngology, Lianyungang First People’s Hospital, Lianyungang, China. jslygdzn@126.com

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• Oncology

OBJECTIVE: Nasopharyngeal carcinoma (NPC) is one of the most ordinary head and neck tumors. This study aims to uncover the role of circ-ABCB10 functions in the progression of NPC.

PATIENTS AND METHODS: The circ-ABCB10 expression was detected in paired NPC patients’ tissue samples and cell lines. The role of circ-ABCB10 in NPC proliferation was identified through proliferation assay, Ethynyl deoxyuridine (EdU) assay and colony formation assay. Next, the role of circ-ABCB10 in NPC metastasis was measured through wound healing assay and transwell assay. Finally, the underlying mechanism was further uncovered through Western blot assay and real-time quantitative polymerase chain reaction (RT-qPCR).

RESULTS: It was found that circ-ABCB10 expression was significantly higher in NPC tissues than that in adjacent samples. In addition, cell proliferation, migration and invasion of NPC were promoted through overexpression of circ-ABCB10 in vitro. Results of further experiments revealed that ROCK1 was upregulated via overexpression of circ-ABCB10 in NPC.

CONCLUSIONS: This study indicated that circ-ABCB10 promoted NPC cell proliferation and metastasis via upregulating ROCK1 in vitro.

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