LncRNA SNHG8 promotes cell migration and invasion in breast cancer cell through miR-634/ZBTB20 axis

D. Fan, B. Qiu, X.-J. Yang, H.-L. Tang, S.-J. Peng, P. Yang, Y.-M. Dong, L. Yang, G.-Q. Bao, H.-D. Zhao

Tangdu Hospital, The Second Affiliated Hospital of Air Force Military Medical University, Xi’an, China. guoqiang@fmmu.edu.cn

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• Oncology

OBJECTIVE: Small nucleolus RNA Host Gene 8 (SNHG8) belongs to a subgroup of long non-coding RNAs. SNHG8 is upregulated in many cancers, such as gastric cancer, liver cancer, and esophageal squamous cell cancer. However, whether SNHG8 is abnormally expressed in breast cancer and its biological functions remain unclear. Therefore, our research intended to determine the expression status of SNHG8 in breast cancer, explore the effects of SNHG8 on the development of breast cancer, and investigate the potential molecular mechanisms in cancer progression.

PATIENTS AND METHODS: The expression levels of SNHG8 were detected in tissue samples and cell lines via qRT-PCR. The effects of SNHG8 on viability of breast cancer cells were detected via CCK-8, EdU, transwell, and flow cytometry analyses.

RESULTS: qRT-PCR results showed that the expression level of SNHG8 was significantly upregulated in tumor tissues and cell lines. Gene functional studies showed that the downregulation of the expression level of SNHG8 significantly inhibited the breast cancer cells migration and invasion, and induced apoptosis. Meanwhile, we found that SNHG8 served as an inhibitor of miR-634 in tumor tissues. SNHG8 may participate in the malignancy of breast cancer by sponging the miR-634 to increase the expression level of ZBTB20.

CONCLUSIONS: The SNHG8-miR-634-ZBTB20 pathway may be a potential target for the treatment of breast cancers.

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