LncRNA UCA1 regulates cervical cancer survival and EMT occurrence by targeting miR-155

T.-J. Yang, L. Wang, Y. Zhang, J.-D. Zheng, L. Liu

Department of Obstetrics and Gynecology, Jinzhou Medical University, Jinzhou, Liaoning, China. ochaktu56@163.com

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• Oncology

OBJECTIVE: Cervical cancer rate is increasing recently. LncRNA UCA1 plays a role in gynecological tumors, but its expression and mechanism in cervical cancer have not yet been elucidated.

PATIENTS AND METHODS: The tumor tissues and adjacent tissues of cervical cancer patients were collected to measure LncRNA UCA1 and miR-155 level by Real-time PCR. The Luciferase report analyzed the relationship between LncRNA UCA1 and miR-155. HeLa cells were separated into NC group, UCA1 siRNA group, UCA1 siRNA + miR-155 inhibitor group followed by analysis of cell proliferation, invasion and migration and EMT-related genes E-cadherin and Vimentin expression by Real time PCR.

RESULTS: UCA1 level was elevated and miR-155 was reduced in cervical cancer tissues with significant differences compared to adjacent tissues (p <0.05). UCA1 was negatively correlated with miR-155 level (p <0.05). Patients with high UCA1 level showed short survival time (p <0.05). Down-regulation of UCA1 can significantly inhibit cell proliferation, migration and invasion. It can also increase E-cadherin expression and decrease Vimentin expression (p <0.05). MiR-155 is a target miRNA of UCA1. MiR-155 inhibitor can significantly reverse UCA1 siRNA’s effect (p <0.05).

CONCLUSIONS: UCA1 expression in cervical cancer is increased and related to patient survival and miR-155 expression is reduced. Lnc-RNA UCA1 regulates EMT occurrence in cervical cancer cells by targeting miR-155.

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