Downregulation of miRNA-429 and upregulation of SOX2 were unfavorable to the prognosis of nasopharyngeal carcinoma

T. Zhang, Y. Tang, Y. Jin, G.-D. Yu

Department of ENT and HN Surgery, Affiliated Hospital of Guizhou Medical University, Guiyang, China. ygd.1224@163.com

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• Oncology

OBJECTIVE: The purpose of this study was to uncover the correlations of expression levels of microRNA-429 (miRNA-429) and SOX2 with clinical parameters and prognosis of nasopharyngeal carcinoma.
PATIENTS AND METHODS: Nasopharyngeal carcinoma patients (n=95) and nasopharyngitis patients (n=95) in the same period were enrolled. The relative levels of miRNA-429 and SOX2 in nasopharyngeal tissues collected from these patients were detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Then, the potential correlation between miRNA-429 and SOX2 was analyzed by Pearson correlation test. Next, the influences of miRNA-429 and SOX2 levels on clinical parameters of nasopharyngeal carcinoma patients were assessed. At last, the factors influencing the prognosis of nasopharyngeal carcinoma were determined using the Cox regression model.
RESULTS: It was found that downregulated miRNA-429 and upregulated SOX2 were observed in nasopharyngeal tissues collected from nasopharyngeal carcinoma patients. MiRNA-429 level was negatively correlated with that of SOX2 in nasopharyngeal carcinoma tissues. In addition, miRNA-429 and SOX2 levels were related to age, tumor differentiation, T stage, N stage, and clinical grade of nasopharyngeal carcinoma patients. Moreover, worse prognosis was seen in nasopharyngeal carcinoma patients expressing low level of miRNA-429 or high level of SOX2. Furthermore, Cox regression analysis showed that T3-T4 stage, moderate to high differentiation, and high level of SOX2 were risk factors, while high level of miRNA-429 was the protective factor for nasopharyngeal carcinoma.
CONCLUSIONS: Downregulation of miRNA-429 and upregulation of SOX2 are unfavorable to the prognosis of nasopharyngeal carcinoma.

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