MiR-885-5p promotes gastric cancer proliferation and invasion through regulating YPEL1

S. Li, M.-Y. Sun, X. Su

Department of Gastroenterology and Hepatology, General Hospital, Tianjin Medical University, Tianjin, P.R. China. tj_lishu@126.com

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• Oncology

OBJECTIVE: Dysregulation of microRNAs (miRNAs) was found to play crucial roles in the carcinogenesis of multiple human cancers. This study was aimed to investigate the biological function of miR-885-5p and associated mechanisms in gastric cancer (GC).
MATERIALS AND METHODS: Reverse Transcription-quantitative Polymerase Chain Reaction was used to measure miR-885-5p level in GC cell lines and normal cell line. The effects of miR-885-5p expression on cell proliferation, colony formation, and invasion were investigated by Cell Counting Kit-8 (CCK-8) assay, colony formation assay, and transwell invasion assay, respectively. Furthermore, Luciferase activity reporter assay and Western blot were conducted to validate Yippee-like-1 (YPEL1) as a direct target of miR-885-5p.
RESULTS: We found that miR-885-5p expression level was elevated in GC cell lines compared with normal cell line. Additionally, the knockdown of miR-885-5p inhibits GC cell proliferation, colony formation, and cell invasion in vitro. Notably, rescue experiments demonstrated that the knockdown of YPEL1 partially reversed the effects of miR-885-5p on GC cell growth and invasion.
CONCLUSIONS: The present study suggested that miR-885-5p regulates GC proliferation, colony formation, and invasion via targeting YPEL1.

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