MiR-506-3p acts as a novel tumor suppressor in prostate cancer through targeting GALNT4

C.-Y. Hu, P. You, J. Zhang, H. Zhang, N. Jiang

Department of Urology, Gongli Hospital Affiliated to The Second Military Medical University, Shanghai, China. sh_huchuanyi@tom.com

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• Oncology

OBJECTIVE: Researches have indicated that microRNA-506-3p (miR-506-3p) was downregulated and functioned as tumor suppressor in cancers. However, the biological role of miR-506-3p in prostate cancer (PCa) remains to be elucidated.

MATERIALS AND METHODS: Expression of miR-506-3p in PCa cell lines was measured by qRT-PCR. Effects of miR-506-3p expression on PCa cell behaviors were investigated with MTT assay, colony formation assay, and transwell invasion assay. Connection of miR-506-3p and N-Acetylgalactosaminyltransferase-4 (GALNT4) was analyzed with luciferase activity reporter assay and Western blot assay.

RESULTS: miR-506-3p expression was downregulated in PCa cell lines. Function studies demonstrated that overexpression of miR-506-3p inhibits PCa tumor progression in vitro. Mechanistic investigations found GALNT4 was a direct target of miR-506-3p. Overexpression of GALNT4 reversed the tumor-suppressive effects of miR-506-3p on PCa cell.

CONCLUSIONS: Our results elucidated genetic silencing of miR-506-3p enhances GALNT4 oncogene expression to accelerate PCa progression.

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