Long non-coding RNA H19 protects acute myocardial infarction through activating autophagy in mice
M. Zhou, Y.-G. Zou, Y.-Z. Xue, X.-H. Wang, H. Gao, H.-W. Dong, Q. Zhang Department of Cardiology, Liaocheng People’s Hospital, Liaocheng, China. qz1878@126.com
OBJECTIVE: We investigate the effect of long non-coding RNA H19 in acute myocardial infarction (AMI) and the underlying mechanism.
MATERIALS AND METHODS: C57BL/6 mice were subjected to AMI and injected with lentivirus pcDNA-H19. After AMI procedures for 3 weeks, cardiac function was detected by echocardiography. The infarct size was stained by triphenyltetrazolium chloride. H19 expression in mice was measured by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). Protein expressions of LC3, Beclin-1, and ATG-7 in mice were measured by Western blot.
RESULTS: Our results indicated that H19 expression was significantly downregulated in the infarcted myocardium. Overexpression of H19 after injection with pcDNA-H19 in mice could reduce infarct size and improve cardiac function through upregulating the ratio of LC3-II/I and expressions of Beclin-1 and ATG-7.
CONCLUSIONS: Overexpression of H19 could protect AMI in mice via activating autophagy.
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To cite this article
M. Zhou, Y.-G. Zou, Y.-Z. Xue, X.-H. Wang, H. Gao, H.-W. Dong, Q. Zhang
Long non-coding RNA H19 protects acute myocardial infarction through activating autophagy in mice
Eur Rev Med Pharmacol Sci
Year: 2018
Vol. 22 - N. 17
Pages: 5647-5651
DOI: 10.26355/eurrev_201809_15831