MicroRNA-155 regulates cervical cancer via inducing Th17/Treg imbalance

Y. Zhang, Z.-C. Wang, Z.-S. Zhang, F. Chen

Department of Gynecology, The People’s Hospital of Weifang, Weifang, China. chenfangwf@sina.com

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• Oncology

OBJECTIVE: To explore the effect of microRNA-155 on cervical cancer and its underlying mechanism.

PATIENTS AND METHODS: Peripheral blood and cervical cancer tissues were collected. We used quantitative Real-time polymerase chain reaction (qRT-PCR) to detect expressions of microRNA-155, SOCS1, Th17-related genes (RORγt, IL-17A, IL-21, and IL-22), and Treg-related genes (foxp3, TGF-β, IL-10, and IL-35) in peripheral blood and cervical cancer tissues. Western blot was used to detect protein expressions of RORγt and foxp3. The proportions of Th17 and Treg cells in CD4+ T cells were measured by flow cytometry. Moreover, IL-17 expression was detected by enzyme-linked immunosorbent assay (ELISA).

RESULTS: MicroRNA-155 was overexpressed in peripheral blood and cervical cancer tissues of patients with cervical cancer compared with those of normal controls. Th17-related transcription factors and cytokines in cervical cancer tissues were remarkably elevated than those of normal controls, including RORγt, IL-17, and IL-6. Treg-related transcription factors and cytokines obtained the similar results. Besides, the proportion of Th17 cells in CD4+ T cells was higher in cervical tissues than that of normal controls. In vitro experiments suggested that overexpressed microRNA-155 can inhibit the expression of target gene SOCS1, promote the differentiation of Th17 and increase levels of IL-17, RORγt, and STAT3.

CONCLUSIONS: MicroRNA-155 is involved in the occurrence and progression of cervical cancer via inhibiting SOSC1 expression and inducing Th17/Treg imbalance.

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