MicroRNA-200c suppresses tumor metastasis in oral squamous carcinoma by inhibiting epithelial-mesenchymal transition

N.-N. Xie, Z.-X. Liu, C. Wu, P.-L. Wang, G.-T. Song, Z. Chen

Hubei Province Key Laboratory of Oral Biomedicine, Wuhan University, Ministry of Education Wuhan, Hubei, P.R. China. chen_zhi1212@163.com

---

• Oncology

OBJECTIVE: To examine the potential mechanisms implicating miR-200c and epithelial-mesenchymal transition (EMT) in oral squamous carcinoma (OSC).

MATERIALS AND METHODS: 32 pairs of OSC tissue samples and matched para-carcinoma normal tissue from patients undergoing routine surgery in the Xuzhou Stomatological Hospital from 2014-2016. HOC313 cells were cultured and transfected with miR-200c mimics and scrambled mimics. Cell migration, invasion assays, Luciferase reporter assay, and Western blot assay were conducted.

RESULTS: miR-200c was downregulated in OSC tissues compared with adjacent normal tissues (n=32). miR-200c knockdown in the human oral cancer cell line HOC313 significantly suppressed cell invasion and migration, indicating the ability to inhibit tumor progression. Luciferase reporter assay indicated that miR-200c directly bound to the 3‘-untranslated regions (3ʹ-UTR) of Zinc finger E-box-binding homeobox (ZEB1) directly. Moreover, miR-200c significantly inhibited HOC313 cell EMT via negatively regulating ZEB1 protein expression.

CONCLUSIONS: MiR-200c plays a pivotal role in controlling OSC metastasis via inhibiting EMT, which provides potential therapeutic targets for OSC.

Free PDF Download