MicroRNA-200c suppressed cervical cancer cell metastasis and growth via targeting MAP4K4

J. Mei, D.-H. Wang, L.-L. Wang, Q. Chen, L.-L. Pan, L. Xia

Department of Gynecology, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, China. 1013756221@qq.com

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• Oncology

OBJECTIVE: To dissect the functioning mode of miR-200c on cervical cancer cell metastasis and growth and provide therapeutic targets for cervical cancer.

PATIENTS AND METHODS: By quantitative Real-time polymerase chain reaction, the miR-200c expression level in 42 pairs of cervical cancer tissue samples and six cervical cancer-derived cell lines were examined. Using miR-200c mimics, we analyzed the effects of miR-200c over-expression on cell proliferation, invasion, and migration. Dual-luciferase activity assay was recruited to examine the potential target gene MAP4K4 that predicted by several databases. Protein level was studied using Western blot.

RESULTS: miR-200c expressed significantly lower in cervical cancer tissue samples and cell lines. And over-expression of miR-200c in cervical cancer cells significantly decreased the cell invasion, migration and proliferation abilities. Dual-luciferase and Western blot confirmed MAP4K4 as a target gene of miR-200c. Furthermore, up-regulation of MAP4K4 counteracted the suppressive effect of miR-200c over-expression on cell growth and metastasis.

CONCLUSIONS: miR-200c could suppress cervical cancer cell proliferation and progression via regulating MAP4K4, which might provide a new target for cervical cancer diagnosis and therapy.

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