TGF-β1 promotes human gastric carcinoma SGC7901 cells invasion by inducing autophagy

J. Shen, D.-S. Zhao, M.-Z. Li

Department of General Surgery, Beijing Chao-Yang Hospital, Capital Medical Univerisity, Beijing, China. limingzhe0213@163.com

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• Oncology

OBJECTIVE: To investigate the role of TGF-b1 in autophagy and invasion ability in human gastric carcinoma cell line SGC7901.

MATERIALS AND METHODS: Cultured SGC7901 cells were treated with different concentrations of TGF-b1 for 24 h. The protein expression levels of autophagy relative marker LC3 and Beclin1 were detected by Western blot. The effect of TGF-b1 on invasion ability of SGC7901 cells was detected with transwell method.

RESULTS: The results demonstrated that TGF-b1 was able to induced autophagy of SGC7901 cells in a dose-dependent manner. Autophagy inhibitor 3-MA could inhibit TGF-b1 upregulated autophagy. Furthermore, TGF-b1 significantly enhances the invasion ability of SGC7901 cells. However, autophagy inhibitor 3-MA could effectively reverse this process.

CONCLUSIONS: TGF-b1 enhances SGC7901 cells migration by inducing autophagy.

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