Does calcium dobesilate protect against intestinal ischemia-reperfusion injury induced in rats?

A. Seker, O. Bardakci, S. Eryilmaz, S. Kocarslan, A. Incebiyik, Y. Yucel, A. Taskin, M. Soyalp, O. Gokalp, A. Uzunkoy

Department of General Surgery, Harran University, Faculty of Medicine, Sanliurfa, Turkey. drsekerahmet@hotmail.com

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• Pharmacology

OBJECTIVE: In this study, we investigated whether the administration of calcium dobesilate (CD) affects oxidative stress markers and histopathological outcomes in a rat model of intestinal ischemia-reperfusion (IR) injury.

MATERIALS AND METHODS: This study was conducted with 30 male Wistar rats. The rats were randomly assigned to three groups as follows: a sham group (n = 10), an IR group (n = 10), and an IR + CD group (n = 10). In the sham group, superior mesenteric artery (SMA) dissection alone was performed during laparotomy. In the IR group, the procedure included SMA occlusion for 60 min, followed by reperfusion for 60 min. In the IR + CD group, CD (100 mg/kg/day) was additionally given for two days before laparotomy by intragastric lavage. In all the rats, 2 ml of blood were drawn, and an ileal segment (approximately 2 cm in size) was removed to evaluate oxidative stress markers. The ileal segment removed was divided into two pieces, and one piece was reserved for histopathological evaluation.

RESULTS: Compared to the other groups, both serum and tissue oxidative stress indices were lower in the IR + CD group. The decrease was due to CD increasing the total antioxidant capacity. Moreover, the histological analysis showed that CD reduced tissue injury.

CONCLUSIONS: CD may exert a protective effect against intestinal IR injury by increasing antioxidant capacity.

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